DNA ligases seal five′-PO4 and 3′-OH polynucleotide finishes by using 3 nucleotidyl transfer ways involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are vital guardians of genomic integrity, and ligase dysfunction underlies human genetic disorder syndromes. Crystal constructions of DNA ligases certain to nucleotide and nucleic acid substrates have illuminated how ligase response chemistry is catalyzed, how ligases realize damaged DNA ends, And exactly how protein area actions and active-site transforming are used to choreograph the tip-joining pathway.
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